I screwed up in the lab for the first time, and it was such a dopey mistake. Okay, so maybe it wasn’t that bad, but I definitely wanted to smack my forehead onto my desk a couple times. I was running a qPCR like I always do, pipetting a billion times into the wells (it seriously takes a good one to one and a half hours), and I put it into the beautiful Applied Biosystems computer. After waiting around for an hour and a half filling up pipette boxes with tiny pipette tips, I took my sunglasses and threw on some sunscreen, and stepped outside to face the blazing hot, sunny weather. If you’re wondering why I had to go out of the Applied Health Sciences building, the qPCR machine is in the UIC College of Medicine building, which is arguably close by…but on a day like this, you’d wish it was closer! The walk was almost unbearable to the point that I could almost hear my skin cells screaming in pain.
Anyway, I got to my machine and selected the wells like I always do, and usually the screen would begin to pop up with pretty colors onto a graph as I clicked from well to well…but today nothing showed up period. After suffering a mere panic attack (I had a couple of those today while I was pipetting into the wells…Margaret, a PhD student working in the lab with me and my lifesaver, sarcastically accused me of coming to the lab drunk since I was so clumsy today, which is obviously false because I don’t drink…no one ever believes me T-T), I immediately called Margaret and she asked if I clicked on a check box labeled “FAM” before starting the qPCR. I couldn’t remember if I did or not, but something strongly tells me that I forgot—and now I am just beating myself up with the fact that I messed up. I would forgive myself and just re-run it again tomorrow if I could, but I had just enough sample to run the qPCR today since I was going to move onto a new project. Sigh. I hope Margaret will save me and magically get the results from what I ran today.
Phew. Rough day and week overall. I had a quiz today for chemistry and I blanked out at the end because we had to find the electron configuration for Gd3+. I mean, seriously, who asks that?! Tutoring at the writing center was really intense because all of the SEWW students were eager to complete their portfolio to get into English 160, but they still lacked a good thesis and they always want help with grammar when that should be the least of their worries.
Luckily work has been treating me well. I’ve been getting a lot of e-mails from the NRHH and RHA committees about updating their website, and coding is one of my favorite things to do! Too bad I am too busy to do it on my own time, but that’s okay.
Sigh. Two more weeks of summer school left. Exam next week, 7-9 page paper due next week, and even more work to do. Each night though, I play on my DS to learn Spanish! I would take it as a class here, but I already got rid of my language requirement with Chinese…and I would rather take an art class than Spanish. I’m thinking about taking Polish or Italian my senior year though. Those would be fun languages to learn. Ah…I can’t wait to have my two weeks of actual summer break! Oh yeah, and supposedly the shirts come in tomorrow. I’ll post up pictures when I can! (:
I want to go to Europe one day…